Purification and solubility of SipC.

<p>SDS-PAGE of SipC inclusion bodies solubilized in 6 M urea and purified by nickel affinity chromatography. Lanes: 1 molecular weight marker, 2-4 supernatants of pellets washes, 5 flowthrough, 6-8 washes, 9-11 elutions, 12 column strip with 0.5 M EDTA, 13 molecular weight marker, 14 insoluble...

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Wedi'i Gadw mewn:

Manylion Llyfryddiaeth
Prif Awdur:	Jacob M. Kroh (22238110) (author)
Awduron Eraill:	Amritangshu Chakravarty (22238113) (author), Supratim Dey (22238116) (author), Roberto N. De Guzman (22238119) (author)
Cyhoeddwyd:	2025
Pynciau:	Biophysics Biochemistry Microbiology Cell Biology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified random coil conformation predominantly alpha helical many bacterial pathogens cause infectious diseases alphafold predictions suggested alpha helical bundles 2d nmr show lack tertiary structures alpha helical proteins two membrane proteins div >< p t3ss translocases ipac secondary structures protein structures dimensional structures thermal denaturation t3ss consists significant amount shigella </ salmonella </ relative sizes minor translocases initiate infection human cells host membrane highest level circular dichroism cd spectroscopy burkholderia </ bacteria use atomic structure
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Purification and solubility of SipC.

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