Purification of recombinant antigens for phage display.

<p>A) Structures of the recombinant proteins GFP-6xHis (GH) and GFP-TDP-43-6xHis (GTH) of 28 kDa and 75 kDa, respectively. B) SDS-PAGE gel stained by Coomassie reagent representing the purification of i) GTH and ii) GH. L: ladder; CE: crude extract of lysate. SN: supernatant of lysate. P: pell...

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Main Author:	Yara Al Ojaimi (12721947) (author)
Other Authors:	Rudolf Hergesheimer (6207317) (author), Anna A. Chami (8725089) (author), Hugo Alarcan (12721953) (author), Johanna Augros (21982577) (author), Audrey Dangoumau (12721950) (author), Shanez Haouari (21982580) (author), Jérôme Bourgeais (19959566) (author), Antoine Lefevre (19490294) (author), Samira Osman (20359173) (author), Patrick Emond (361155) (author), Patrick Vourc’h (826890) (author), Christian R. Andres (8450154) (author), Philippe Corcia (136280) (author), Olivier Herault (439477) (author), Pierre Martineau (6238) (author), Débora Lanznaster (6567578) (author), Hélène Blasco (361150) (author)
Published:	2025
Subjects:	Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Cancer Biological Sciences not elsewhere classified neurological disorders marked induced metabolomic alterations chain variable fragment new scfv intrabody length wt tdp div >< p terminal fragment vitro .</ untargeted high two scfvs throughput screen termed intrabodies silico </ proteasomal degradation particularly relating normal function mislocalization disrupts lipid metabolism kda c insoluble 35 human wttdp human full findings offer epitope mapping cytoplasmic tdp cellular model abnormal accumulation 3d structure
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Purification of recombinant antigens for phage display.

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