Purification and titers determination of RABV G-pseudotyped recombinant lentivirus.

Purification and titers determination of RABV G-pseudotyped recombinant lentivirus.

<p>After transfection with plasmids for 48 hours, purify the viral particles packaged by HEK293T cells using sucrose density gradient centrifugation and measure their concentration by qPCR.</p>

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主要作者:	Lifang Shuai (311262) (author)
其他作者:	Mingyu Xu (1894687) (author), Nana Pei (504330) (author), Cheng Zhu (138742) (author), Marwa Belghait (22127370) (author), Zhiming Hu (3112455) (author), Jinlong Li (388896) (author), Hongwei Li (17402) (author), Yingying Mao (6389225) (author), Hongyan Du (305605) (author)
出版:	2025
主题:	Medicine Microbiology Genetics Biotechnology Ecology Immunology Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified strong immune responses require multiple doses pseudotyped lentiviral vectors pseudotyped lentiviral particle neutralizing antibody response inducing protective antibodies efficient gene delivery xlink "> rabies virus particles production new vaccine development rabies virus glycoprotein g show promise g ), essential rabv ), remains rabies virus odn adjuvant mice showed immunization tests high titers exposure prophylaxis egfp vaccine among rabv 10 weeks
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