Apoptosis of MDA-MB-231 cells exposed to tick saliva.

<p>Protein and non-protein fractions of tick saliva were diluted in DMEM at the indicated concentrations and then added to subconfluent MDA-MB-231 cells. PBS-treated cells were used as control. After 72h of incubation, cells were loaded with Hoechst to stain nuclei. Apoptosis was evaluated by...

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Hlavní autor:	Ana Carolina Prado Sousa (22265110) (author)
Další autoři:	Mario Durán-Prado (637398) (author), Margarita Villar (420749) (author), Almudena González-García (16619940) (author), Matias Pablo Juan Szabó (6048464) (author), José de la Fuente (35632) (author)
Vydáno:	2025
Témata:	Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Pharmacology Biotechnology Immunology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified propidium iodide assays potential therapeutic applications potential anticancer properties novel therapeutic agents including anticancer properties identifying active biomolecules hoechst staining revealed hoechst staining quantified based fluorescence method hacat control cells cells per well evaluated using hoescht breast cancer cells 231 cancer cells protein bioactive molecules ml ), whereas inhibit cell attachment div >< p tick saliva components protein fractions showed rhipicephalus sanguineus </ decreasing cell viability tick saliva protein cancer cells cell attachment tick saliva protein fractions sanguineus </ cell viability assessed using protein fraction vitro </ sculptum </ parvum </ americanum </ amblyomma </ total number slight increase significant reduction proteomics analysis highest concentration future studies findings provide chemical composition 10 µg 1 µg 1 %)
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Apoptosis of MDA-MB-231 cells exposed to tick saliva.

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