Evaluation of long-term preOst1-DNase1 expression.

<p>Prolonged expression of (A) murine and (B) human preOst1-DNase1 over 72 hours with (+) or without (-) daily application of 2.5% (v/v) MeOH with no ongoing DNase1 expression as evaluated by silver gel analysis of SN combined with (A) HCA or (B) DPZ below. Cells were incubated in 1% (w/v) PM...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Jan-Ole Krischek (21213122) (author)
مؤلفون آخرون:	Hans Georg Mannherz (10226546) (author), Markus Napirei (10226549) (author)
منشور في:	2025
الموضوعات:	Biochemistry Genetics Molecular Biology Biotechnology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified systemic lupus erythematosus rough endoplasmic reticulum related waste disposal major endonuclease involved important therapeutic biologic higher specific activity chinese hamster ovary also detected previously 19 associated microangiopathy saccharomyces cerevisiae </ pichia pastoris </ div >< p using mdnase1 expression recombinant murine dnase1 pastoris </ recombinant dnase1 murine dnase1 translational import since deoxyribonuclease salient feature rhdnase1 produced rhdnase1 expressed rhdnase1 due rhdnase1 ). pulmozyme ®, prolonged persistence orthologues share oligosaccharyltransferase complex native conformation methanol supply main cause lower inhibition improvements resulted impaired cleavage high structural hdnase1 resulted hdnase1 fourfold hdnase1 expression functional homology extracellular chromatin expression difference cultivation temperature concerning functionality comparative expression codon usage cell density biochemical effectivity =&# 8201 24 hours
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Evaluation of long-term preOst1-DNase1 expression.

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