Representative movie of a VAMP7-pHluorin transfected RPE-1 cell in TIRFM.

Representative movie of a VAMP7-pHluorin transfected RPE-1 cell in TIRFM.

<p>White squares highlight some of the exocytosis events characterized by a sudden apparition of a bright spot and then the 2D diffusion of the signal. Time code is in minute:second format.</p> <p>(MP4)</p>

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Bibliographic Details
Main Author:	Hugo Lachuer (22430751) (author)
Other Authors:	Emmanuel Moebel (22430754) (author), Anne-Sophie Macé (18135432) (author), Arthur Masson (10816460) (author), Kristine Schauer (529393) (author), Charles Kervrann (242142) (author)
Published:	2025
Subjects:	Biochemistry Cell Biology Space Science Biological Sciences not elsewhere classified recently shown promise net originally designed deep learning model deep learning approaches annotated training datasets unsupervised conventional methods dynamic processes obtained dynamic exocytosis events xlink "> segmentation unseen experimental conditions deep learning detection custom user data 3d noisy cryo 3d images whereas dynamic processes experimental conditions 12000 events tested methods temporal series static objects source software paramount importance napari plugin made transparent less explored lapse acquisitions imaged reporter greater plasticity fluorescence microscopy electron tomography drug treatments cell line cell imaging biological objects apparent plasticity also exhibited adapted deepfinder accelerate analysis 60 cells
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