Representative movie of a VAMP7-pHluorin transfected RPE-1 cell in TIRFM.

Representative movie of a VAMP7-pHluorin transfected RPE-1 cell in TIRFM.

<p>White squares highlight some of the exocytosis events characterized by a sudden apparition of a bright spot and then the 2D diffusion of the signal. Time code is in minute:second format.</p> <p>(MP4)</p>

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Hugo Lachuer (22430751) (author)
مؤلفون آخرون: Emmanuel Moebel (22430754) (author), Anne-Sophie Macé (18135432) (author), Arthur Masson (10816460) (author), Kristine Schauer (529393) (author), Charles Kervrann (242142) (author)
منشور في: 2025
الموضوعات:
Biochemistry
Cell Biology
Space Science
Biological Sciences not elsewhere classified
recently shown promise
net originally designed
deep learning model
deep learning approaches
annotated training datasets
unsupervised conventional methods
dynamic processes obtained
dynamic exocytosis events
xlink "> segmentation
unseen experimental conditions
deep learning detection
custom user data
3d noisy cryo
3d images whereas
dynamic processes
experimental conditions
12000 events
tested methods
temporal series
static objects
source software
paramount importance
napari plugin
made transparent
less explored
lapse acquisitions
imaged reporter
greater plasticity
fluorescence microscopy
electron tomography
drug treatments
cell line
cell imaging
biological objects
apparent plasticity
also exhibited
adapted deepfinder
accelerate analysis
60 cells
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