Purification of IpaC CTD (residues 173-363).

<p>(A) First purification of IpaC CTD by nickel affinity chromatography. Lanes: 1 molecular weight marker, 2 supernatant, 3 flowthrough, 4, 5. washes, 6-8. Elution of His-GB1-TEV- IpaC CTD *. (B) Second purification of IpaC CTD by nickel affinity chromatography after TEV protease digestion. La...

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Váldodahkki:	Jacob M. Kroh (22238110) (author)
Eará dahkkit:	Amritangshu Chakravarty (22238113) (author), Supratim Dey (22238116) (author), Roberto N. De Guzman (22238119) (author)
Almmustuhtton:	2025
Fáttát:	Biophysics Biochemistry Microbiology Cell Biology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified random coil conformation predominantly alpha helical many bacterial pathogens cause infectious diseases alphafold predictions suggested alpha helical bundles 2d nmr show lack tertiary structures alpha helical proteins two membrane proteins div >< p t3ss translocases ipac secondary structures protein structures dimensional structures thermal denaturation t3ss consists significant amount shigella </ salmonella </ relative sizes minor translocases initiate infection human cells host membrane highest level circular dichroism cd spectroscopy burkholderia </ bacteria use atomic structure
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Purification of IpaC CTD (residues 173-363).

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