Threshold cutoff values required to correctly call known-positive samples.

For each group, the percentage of correctly-called known-“Ca. Liberibacter asiaticus” (CLas)-positive detection results in samples are plotted against cycle number threshold values between 16 and 40 cycles. As the value increases, the number of known-po...

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Bibliographic Details
Main Author:	Nathaniel Ponvert (17184784) (author)
Other Authors:	Frank Byrne (21354976) (author), Monique J. Rivera (17204545) (author), Timo Rohula (21354979) (author), Sandra Olkowski (2137909) (author), Heshani De Silva Weligodage (21354982) (author), Neil McRoberts (4716426) (author), Judith K. Brown (11722838) (author)
Published:	2025
Subjects:	Microbiology Biotechnology Ecology Sociology Plant Biology Biological Sciences not elsewhere classified vector diseases affecting time quantitative pcr ten per tube psyllid developmental stage droplet digital pcr california using real 4 – 5 liberibacter asiaticus ’ genome load peaking div >< p asian citrus psyllid diaphorina citri </ california citrus groves adult acp might adult acp age citrus greening disease las detection correlated consistent qpcr detection citrus groves adult acp grove ’ genome copies citrus trees citrus industry >< b candidatus </ c </ acp adults temporal detection reliable detection las detection early detection detection rates uneven distribution symptomatic phase stable throughout sample size results indicated qpcr amplification potentially misrepresenting optimized scouting optimize approaches optimal number long pre las throughout las status infected trees field populations dna isolation destructive bacterial
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Threshold cutoff values required to correctly call known-positive samples.

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