Trypsin treatment increases ACE2-dependent cell entry and ACE2 binding by bat CoV spikes. scVSVs bearing the spikes of SHC014-CoV (WT or Y623H) were mixed with 200 µg/mL of trypsin and added onto Vero cells.

<p>At 16–18 h post-infection, infection levels were scored by eGFP expression (average±SD. n = 6 from 3 independent experiments). Infectivity values were normalized to no-trypsin controls. Groups (no trypsin vs. trypsin treatment) were compared with unpaired t-test with Welch’s correction, ns...

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Bibliographic Details
Main Author:	Alexandra L. Tse (20235212) (author)
Other Authors:	Gorka Lasso (215085) (author), Jacob Berrigan (11052951) (author), Jason S. McLellan (8816639) (author), Kartik Chandran (134142) (author), Emily Happy Miller (10652108) (author)
Published:	2025
Subjects:	Neuroscience Evolutionary Biology Cancer Infectious Diseases Virology significant zoonotic threat mammalian respiratory tract host protease barrier genetic pathways associated even initially replace poor entry activity key viral adaptation entry could precede alters spike dynamics increased spike opening enhances spike opening xlink "> sars s1 &# 8211 loop &# 8217 enhance spike cleavage opening substitutions arose bat sarbecovirus wiv1 cov spikes could viral entry &# 8217 opening substitutions s2 cleavage s1 subunit cell entry &# 8216 type spike spike resembling successful isolation single amino s2 boundary rhinolophid bats pseudotype system previously showed previously identified oral lifestyle natural hosts like betacoronaviruses incompletely understood immune evasion findings suggest currently recognized cov using cov bears conformational constraints cell culture broader property binding domain bats pose bat sarbecoviruses adaptive mutation
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Trypsin treatment increases ACE2-dependent cell entry and ACE2 binding by bat CoV spikes. scVSVs bearing the spikes of SHC014-CoV (WT or Y623H) were mixed with 200 µg/mL of trypsin and added onto Vero cells.

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