Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i>
<p>A) markers A<sub>a</sub> and B<sub>a</sub> were outputted by WheresWalker and used to genotype <i>arches</i> mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highl...
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2025
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| Summary: | <p>A) markers A<sub>a</sub> and B<sub>a</sub> were outputted by WheresWalker and used to genotype <i>arches</i> mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highlighted) products are as indicated. B) Points representing A<sub>a</sub> (red) and B<sub>a</sub> (blue) marker locations and horizontal lines representing the estimated distance to the causative mutation are overlaid on the SNP index for the <i>arches</i> interval (chromosome 1: 2.46-13.86 Mb). The vertical dashed line indicates the location of <i>mttp</i>, the causative locus. C) quantification of ApoBb.1-nanoluciferase levels at 3 dpf. Mean ± standard deviation, N = 4-5 clutches, n = 2-8 animals/datapoint. P < 0.05 by two-way ANOVA with Geisser-Greenhouse correction and Tukey’s multiple comparisons test. * vs. respective wild-type, ^ vs <i>c655</i>/ + , $ vs <i>c655</i>/c655, # vs <i>stl</i>/stl. D) Images of whole-animal ApoBb.1-nanoluciferase distribution in <i>arches</i> and <i>stl</i> mutants at 3 dpf. E) Brightfield images of mutant and wild-type animals from 2-4 dpf. For D and E, scale bar represents 1 mm.</p> |
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