Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i>
<p>A) markers A<sub>a</sub> and B<sub>a</sub> were outputted by WheresWalker and used to genotype <i>arches</i> mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highl...
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2025
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| _version_ | 1852018495850545152 |
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| author | McKenna Feltes (11242085) |
| author2 | Aleksey V. Zimin (8363328) Sofia Angel (21714119) Nainika Pansari (21714122) Monica R. Hensley (3133671) Jennifer L. Anderson (15991713) Meng-Chieh Shen (4608190) Mackenzie Klemek (21714125) Yi Shen (152956) Vighnesh S. Ginde (21714128) Hannah Kozan (21714131) Nhan V. Le (21714134) Vivian P. Truong (21714137) Meredith H. Wilson (6816489) Steven L. Salzberg (8363331) Steven A. Farber (9217109) |
| author2_role | author author author author author author author author author author author author author author author |
| author_facet | McKenna Feltes (11242085) Aleksey V. Zimin (8363328) Sofia Angel (21714119) Nainika Pansari (21714122) Monica R. Hensley (3133671) Jennifer L. Anderson (15991713) Meng-Chieh Shen (4608190) Mackenzie Klemek (21714125) Yi Shen (152956) Vighnesh S. Ginde (21714128) Hannah Kozan (21714131) Nhan V. Le (21714134) Vivian P. Truong (21714137) Meredith H. Wilson (6816489) Steven L. Salzberg (8363331) Steven A. Farber (9217109) |
| author_role | author |
| dc.creator.none.fl_str_mv | McKenna Feltes (11242085) Aleksey V. Zimin (8363328) Sofia Angel (21714119) Nainika Pansari (21714122) Monica R. Hensley (3133671) Jennifer L. Anderson (15991713) Meng-Chieh Shen (4608190) Mackenzie Klemek (21714125) Yi Shen (152956) Vighnesh S. Ginde (21714128) Hannah Kozan (21714131) Nhan V. Le (21714134) Vivian P. Truong (21714137) Meredith H. Wilson (6816489) Steven L. Salzberg (8363331) Steven A. Farber (9217109) |
| dc.date.none.fl_str_mv | 2025-07-14T17:33:09Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pgen.1011702.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Recombinant_mapping_narrows_region_of_interest_to_identify_a_loss-of-function_allele_of_i_mttp_i_/29563811 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Microbiology Genetics Neuroscience Evolutionary Biology Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Mathematical Sciences not elsewhere classified supports positional cloning method using mutants forward genetic screening allelic frequency calculations whereswalker correctly mapped sequencing approach based previously unappreciated apob previously published mutation div >< p sequencing algorithm powerful approach describe whereswalker slc3a2a </ potential candidates mttp </ modifying locus mia2 </ lipoprotein metabolism key step gene causing drastically reduces assign functions 1 </ |
| dc.title.none.fl_str_mv | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A) markers A<sub>a</sub> and B<sub>a</sub> were outputted by WheresWalker and used to genotype <i>arches</i> mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highlighted) products are as indicated. B) Points representing A<sub>a</sub> (red) and B<sub>a</sub> (blue) marker locations and horizontal lines representing the estimated distance to the causative mutation are overlaid on the SNP index for the <i>arches</i> interval (chromosome 1: 2.46-13.86 Mb). The vertical dashed line indicates the location of <i>mttp</i>, the causative locus. C) quantification of ApoBb.1-nanoluciferase levels at 3 dpf. Mean ± standard deviation, N = 4-5 clutches, n = 2-8 animals/datapoint. P < 0.05 by two-way ANOVA with Geisser-Greenhouse correction and Tukey’s multiple comparisons test. * vs. respective wild-type, ^ vs <i>c655</i>/ + , $ vs <i>c655</i>/c655, # vs <i>stl</i>/stl. D) Images of whole-animal ApoBb.1-nanoluciferase distribution in <i>arches</i> and <i>stl</i> mutants at 3 dpf. E) Brightfield images of mutant and wild-type animals from 2-4 dpf. For D and E, scale bar represents 1 mm.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_fea6bacfa9b5d9c2d922ead27d3e6db5 |
| identifier_str_mv | 10.1371/journal.pgen.1011702.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29563811 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i>McKenna Feltes (11242085)Aleksey V. Zimin (8363328)Sofia Angel (21714119)Nainika Pansari (21714122)Monica R. Hensley (3133671)Jennifer L. Anderson (15991713)Meng-Chieh Shen (4608190)Mackenzie Klemek (21714125)Yi Shen (152956)Vighnesh S. Ginde (21714128)Hannah Kozan (21714131)Nhan V. Le (21714134)Vivian P. Truong (21714137)Meredith H. Wilson (6816489)Steven L. Salzberg (8363331)Steven A. Farber (9217109)MedicineMicrobiologyGeneticsNeuroscienceEvolutionary BiologyInfectious DiseasesVirologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedMathematical Sciences not elsewhere classifiedsupports positional cloningmethod using mutantsforward genetic screeningallelic frequency calculationswhereswalker correctly mappedsequencing approach basedpreviously unappreciated apobpreviously published mutationdiv >< psequencing algorithmpowerful approachdescribe whereswalkerslc3a2a </potential candidatesmttp </modifying locusmia2 </lipoprotein metabolismkey stepgene causingdrastically reducesassign functions1 </<p>A) markers A<sub>a</sub> and B<sub>a</sub> were outputted by WheresWalker and used to genotype <i>arches</i> mutants in order to identify recombinants. M and F denote male and female parents, respectively. PCR product sizes for wild-type and mutant (highlighted) products are as indicated. B) Points representing A<sub>a</sub> (red) and B<sub>a</sub> (blue) marker locations and horizontal lines representing the estimated distance to the causative mutation are overlaid on the SNP index for the <i>arches</i> interval (chromosome 1: 2.46-13.86 Mb). The vertical dashed line indicates the location of <i>mttp</i>, the causative locus. C) quantification of ApoBb.1-nanoluciferase levels at 3 dpf. Mean ± standard deviation, N = 4-5 clutches, n = 2-8 animals/datapoint. P < 0.05 by two-way ANOVA with Geisser-Greenhouse correction and Tukey’s multiple comparisons test. * vs. respective wild-type, ^ vs <i>c655</i>/ + , $ vs <i>c655</i>/c655, # vs <i>stl</i>/stl. D) Images of whole-animal ApoBb.1-nanoluciferase distribution in <i>arches</i> and <i>stl</i> mutants at 3 dpf. E) Brightfield images of mutant and wild-type animals from 2-4 dpf. For D and E, scale bar represents 1 mm.</p>2025-07-14T17:33:09ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pgen.1011702.g005https://figshare.com/articles/figure/Recombinant_mapping_narrows_region_of_interest_to_identify_a_loss-of-function_allele_of_i_mttp_i_/29563811CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/295638112025-07-14T17:33:09Z |
| spellingShingle | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> McKenna Feltes (11242085) Medicine Microbiology Genetics Neuroscience Evolutionary Biology Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Mathematical Sciences not elsewhere classified supports positional cloning method using mutants forward genetic screening allelic frequency calculations whereswalker correctly mapped sequencing approach based previously unappreciated apob previously published mutation div >< p sequencing algorithm powerful approach describe whereswalker slc3a2a </ potential candidates mttp </ modifying locus mia2 </ lipoprotein metabolism key step gene causing drastically reduces assign functions 1 </ |
| status_str | publishedVersion |
| title | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| title_full | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| title_fullStr | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| title_full_unstemmed | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| title_short | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| title_sort | Recombinant mapping narrows region of interest to identify a loss-of-function allele of <i>mttp.</i> |
| topic | Medicine Microbiology Genetics Neuroscience Evolutionary Biology Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Mathematical Sciences not elsewhere classified supports positional cloning method using mutants forward genetic screening allelic frequency calculations whereswalker correctly mapped sequencing approach based previously unappreciated apob previously published mutation div >< p sequencing algorithm powerful approach describe whereswalker slc3a2a </ potential candidates mttp </ modifying locus mia2 </ lipoprotein metabolism key step gene causing drastically reduces assign functions 1 </ |