The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol
Listeria monocytogenes biofilms present a significant challenge in the food industry. This study explores the impact of different acidic conditions of culture media and food matrices on the development and removal of biofilms developed on stainless steel surfaces by wild-type (WT) L. monocytogenes s...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , |
| Format: | article |
| Published: |
2024
|
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1016/j.ijfoodmicro.2024.110676 https://www.sciencedirect.com/science/article/pii/S016816052400120X http://hdl.handle.net/10576/55218 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1857415084478824448 |
|---|---|
| author | Jina, Yammine |
| author2 | Doulgeraki, Agapi I. O'Byrne, Conor P. Gharsallaoui, Adem Chihib, Nour-Eddine Karam, Layal |
| author2_role | author author author author author |
| author_facet | Jina, Yammine Doulgeraki, Agapi I. O'Byrne, Conor P. Gharsallaoui, Adem Chihib, Nour-Eddine Karam, Layal |
| author_role | author |
| dc.creator.none.fl_str_mv | Jina, Yammine Doulgeraki, Agapi I. O'Byrne, Conor P. Gharsallaoui, Adem Chihib, Nour-Eddine Karam, Layal |
| dc.date.none.fl_str_mv | 2024-05-20T11:31:08Z 2024-05-02 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1016/j.ijfoodmicro.2024.110676 Yammine, J., Doulgeraki, A. I., O'Byrne, C. P., Gharsallaoui, A., Chihib, N. E., & Karam, L. (2024). The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol. International Journal of Food Microbiology, 416, 110676. 0168-1605 https://www.sciencedirect.com/science/article/pii/S016816052400120X http://hdl.handle.net/10576/55218 416 1879-3460 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Listeria monocytogenes Biofilms pH Food Nanoencapsulated carvacrol |
| dc.title.none.fl_str_mv | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Listeria monocytogenes biofilms present a significant challenge in the food industry. This study explores the impact of different acidic conditions of culture media and food matrices on the development and removal of biofilms developed on stainless steel surfaces by wild-type (WT) L. monocytogenes strains as well as in two mutant derivatives, ΔsigB and ΔagrA, that have defects in the general stress response and quorum sensing, respectively. Additionally, the study investigates the efficacy of nanoencapsulated carvacrol as an antimicrobial against L. monocytogenes biofilms developed in Tryptic Soy Broth (TSB) culture media acidified to different pH conditions (3.5, 4.5, 5.5, 6.5), and in food substrates (apple juice, strained yogurt, vegetable soup, semi-skimmed milk) having the same pH levels. No biofilm formation was observed for all L. monocytogenes strains at pH levels of 3.5 and 4.5 in both culture media and food substrates. However, at pH 5.5 and 6.5, increased biofilm levels were observed in both the culture media and food substrates, with the WT strain showing significantly higher biofilm formation (3.04–6.05 log CFU cm−2) than the mutant strains (2.30–5.48 log CFU cm−2). For both applications, the nanoencapsulated carvacrol demonstrated more potent antimicrobial activity against biofilms developed at pH 5.5 with 2.23 to 3.61 log reductions, compared to 1.58–2.95 log reductions at pH 6.5, with mutants being more vulnerable in acidic environments. In food substrates, nanoencapsulated carvacrol induced lower log reductions (1.58–2.90) than the ones in TSB (2.02–3.61). These findings provide valuable insights into the impact of different acidic conditions on the development of L. monocytogenes biofilms on stainless steel surfaces and the potential application of nanoencapsulated carvacrol as a biofilm control agent in food processing environments. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | qu_0829e70d784a633b67a2917bfcfc7c6a |
| identifier_str_mv | Yammine, J., Doulgeraki, A. I., O'Byrne, C. P., Gharsallaoui, A., Chihib, N. E., & Karam, L. (2024). The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol. International Journal of Food Microbiology, 416, 110676. 0168-1605 416 1879-3460 |
| language_invalid_str_mv | en |
| network_acronym_str | qu |
| network_name_str | Qatar University repository |
| oai_identifier_str | oai:qspace.qu.edu.qa:10576/55218 |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Elsevier |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| spelling | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrolJina, YammineDoulgeraki, Agapi I.O'Byrne, Conor P.Gharsallaoui, AdemChihib, Nour-EddineKaram, LayalListeria monocytogenesBiofilmspHFoodNanoencapsulated carvacrolListeria monocytogenes biofilms present a significant challenge in the food industry. This study explores the impact of different acidic conditions of culture media and food matrices on the development and removal of biofilms developed on stainless steel surfaces by wild-type (WT) L. monocytogenes strains as well as in two mutant derivatives, ΔsigB and ΔagrA, that have defects in the general stress response and quorum sensing, respectively. Additionally, the study investigates the efficacy of nanoencapsulated carvacrol as an antimicrobial against L. monocytogenes biofilms developed in Tryptic Soy Broth (TSB) culture media acidified to different pH conditions (3.5, 4.5, 5.5, 6.5), and in food substrates (apple juice, strained yogurt, vegetable soup, semi-skimmed milk) having the same pH levels. No biofilm formation was observed for all L. monocytogenes strains at pH levels of 3.5 and 4.5 in both culture media and food substrates. However, at pH 5.5 and 6.5, increased biofilm levels were observed in both the culture media and food substrates, with the WT strain showing significantly higher biofilm formation (3.04–6.05 log CFU cm−2) than the mutant strains (2.30–5.48 log CFU cm−2). For both applications, the nanoencapsulated carvacrol demonstrated more potent antimicrobial activity against biofilms developed at pH 5.5 with 2.23 to 3.61 log reductions, compared to 1.58–2.95 log reductions at pH 6.5, with mutants being more vulnerable in acidic environments. In food substrates, nanoencapsulated carvacrol induced lower log reductions (1.58–2.90) than the ones in TSB (2.02–3.61). These findings provide valuable insights into the impact of different acidic conditions on the development of L. monocytogenes biofilms on stainless steel surfaces and the potential application of nanoencapsulated carvacrol as a biofilm control agent in food processing environments.This work was funded by the E-COST-GRANT-CA18113 [66ea6e99], “Understanding and exploiting the impacts of low pH on microorganisms” (EuroMicropH). The open access funding was provided by the Qatar National Library.Elsevier2024-05-20T11:31:08Z2024-05-02Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ijfoodmicro.2024.110676Yammine, J., Doulgeraki, A. I., O'Byrne, C. P., Gharsallaoui, A., Chihib, N. E., & Karam, L. (2024). The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol. International Journal of Food Microbiology, 416, 110676.0168-1605https://www.sciencedirect.com/science/article/pii/S016816052400120Xhttp://hdl.handle.net/10576/552184161879-3460enhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:qspace.qu.edu.qa:10576/552182025-05-25T09:45:15Z |
| spellingShingle | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol Jina, Yammine Listeria monocytogenes Biofilms pH Food Nanoencapsulated carvacrol |
| status_str | publishedVersion |
| title | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| title_full | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| title_fullStr | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| title_full_unstemmed | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| title_short | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| title_sort | The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol |
| topic | Listeria monocytogenes Biofilms pH Food Nanoencapsulated carvacrol |
| url | http://dx.doi.org/10.1016/j.ijfoodmicro.2024.110676 https://www.sciencedirect.com/science/article/pii/S016816052400120X http://hdl.handle.net/10576/55218 |